Q) What does this mean because of the requirement of the origin of replication that a piece of - Biology - Molecular Basis of Inheritance

Question

Q) What does this mean?
because of the requirement of the origin of replication that a
piece of DNA if needed to be propagated during recombinant DNA
procedures, requires a vector The vectors provide the origin of
replication

Mushtaq · Accepted Answer

Dear student

Please refer the solution below for the said query

It means that an orgin of replication is needed to produce multiple
copies of a desired gene (a piece of DNA) during recombinant DNA
technology The origin of replication is a region where replication
of DNA is started by the enzymes

The desired gene (a piece of DNA) does not have its own origin of
replication Hence, desired gene is ligated with vector DNA like
plasmid of bacteria containing origin of replication for producing
its multiple copies in any host organism (say E coli)

Hope this information clears your doubt about the topic

Regards

Q). What does this mean? because of the requirement of the origin of replication that a piece of DNA if needed to be propagated during recombinant DNA procedures, requires a vector. The vectors provide the origin of replication.

Q). What does this mean?
because of the requirement of the origin of replication that a piece of DNA if needed to be propagated during recombinant DNA procedures, requires a vector. The vectors provide the origin of replication.