to transform a bacterial cell with r dna what is required-
90 c temp
divalent calcium
selectable marker
restriction enzyme
choose any 1; correctly
Dear student,
90oC temp is required for DNA denaturation in a PCR (Polymerase Chain Reaction) which is used to amplify the gene of interest.
Selectable marker after the DNA has been introduced into the bacterial cells (transformation achieved), selectable marker helps us to differentiate the transformed cells from the non-transformed ones.
Divalent calcium is used to make a cell competent so that it can easily take up the foreign DNA by creating pores in cell wall of the bacterial cell so that it can undergo transformation.
Restriction enzymes are required to cut out the gene of interest from DNA. This is then ligated to the vector to obtain the Recombinant DNA
Thus, out of the 4 options, Divalent calcium would be the most appropriate answer since bacterial cells undergo transformation only after becoming competent (by addition of divalent calcium ions).
Regards
90oC temp is required for DNA denaturation in a PCR (Polymerase Chain Reaction) which is used to amplify the gene of interest.
Selectable marker after the DNA has been introduced into the bacterial cells (transformation achieved), selectable marker helps us to differentiate the transformed cells from the non-transformed ones.
Divalent calcium is used to make a cell competent so that it can easily take up the foreign DNA by creating pores in cell wall of the bacterial cell so that it can undergo transformation.
Restriction enzymes are required to cut out the gene of interest from DNA. This is then ligated to the vector to obtain the Recombinant DNA
Thus, out of the 4 options, Divalent calcium would be the most appropriate answer since bacterial cells undergo transformation only after becoming competent (by addition of divalent calcium ions).
Regards