Genetic engineering is a technique for artificially and deliberately modifying DNA (genes) to suit human needs. It includes techniques like creating recombinant , gene cloning and gene transfer.
Steps in genetic engineering:
1. Identification and isolation of genetic material.
2. Cutting of the DNA at specific locations using restriction enzymes.
3. Amplification of the desired gene using Polymerase Chain reaction.
4. Introduction of gene of interest into host cell or organism.
5.The gene is cloned and the desired gene product the recombinant protein is obtained.
6. Downstream processing is done which includes processing like and separation purification
