what is meant by acid soluble pool?
when a living tissue is grinded with trichloroacetic acid,a thick slurry is formed.This slurry when strained by cottonor cheesecloth gives 2 fractions.one is the filtrate which is called acid soluble pool where 1000 of organic compounds are found.The other fractionis called acid insoluble pool where compounds like proteins,nucleic acid,polysaccaride,nucleic acid is found.
Grind any living tissue (a vegetable or a piece of liver, etc.) in trichloroacetic acid (Cl3CCOOH) using a mortar and a pestle. We obtain thick slurry. Strain this through a cheesecloth or cotton to obtain two fractions. One is called the filtrate or more technically, the acid-soluble pool, and the second, the retentate or the acid-insoluble fraction. Scientists have found thousands of organic compounds in the acid-soluble pool.