what is the role of PCR in molecular diagnosis?
PCR ( Polymerase Chain Reaction) is a molecular technique that basically helps in amplifying the specific section of DNA to many times with the use of specific primers. This technique has various applications among which molecular diagnosis is one. PCR by amplifying the DNA to many times helps in detection of even minute quantities of nucleic acids( DNA ) easily. So also helps in early and highly sensitive detection of disease causing agents in body by amplifying their specific DNA.
For example:
PCR helps in detection of infectious agents by virtue of specific genes. It helps in early detection of HIV (causing AIDS), even before the onset of disease. The specific primers to the targeted sequences in the DNA of a virus are used to amplify the viruses nucleic acid after it has been extracted from the blood. Then, the amplified viral DNA can be quantified.
PCR is also used to perform to diagnosis of cancers like leukemia and lymphomas. PCR in this case works by detecting translocation, gene mutation in genomic DNA of malignant cells of suspected cancer patients. PCR can amplify a specific DNA fragment with mutation by using specific primers and the result of PCR can be than detected on the gel with electrophoresis.
For example:
PCR helps in detection of infectious agents by virtue of specific genes. It helps in early detection of HIV (causing AIDS), even before the onset of disease. The specific primers to the targeted sequences in the DNA of a virus are used to amplify the viruses nucleic acid after it has been extracted from the blood. Then, the amplified viral DNA can be quantified.
PCR is also used to perform to diagnosis of cancers like leukemia and lymphomas. PCR in this case works by detecting translocation, gene mutation in genomic DNA of malignant cells of suspected cancer patients. PCR can amplify a specific DNA fragment with mutation by using specific primers and the result of PCR can be than detected on the gel with electrophoresis.