Differentiate clonning vector and expression vector.
Solution:
Characteristics | Cloning Vector | Expression vector |
Definition | It can be described as the small DNA fragment that is responsible for maintaining stability inside the host cell. It also leads to creation of multiple gene copies of interest inside the host cell. | It can be described as an circular extra chromosomal, double-stranded DNA. It also has self-replicating properties. |
Features | It is made up of an ori, restriction sites, reporter gene and a specific antibiotic resistance site. | It is made up of enhancers, promoters, termination sequences, transcription and translation initiation site and more. |
Types | Plasmids, YACs, BACs, cosmids and more. | Plasmid |
Use | It is used to introduce foreign gene into a host. | It is used to express the introduced gene by synthesizing a relevant protein. |