DNA sequences were then arranged based on some overlapping regions present on them. What is meant by overlapping regions?

Dear student,Overlapping genes are defined as a pair of adjacent genes whose coding regions are partially overlapping. In other words, a single stretch of DNA codes for portions of two separate proteins. Such an arrangement of genetic code is ubiquitous. Many overlapping genes have been identified in the genomes of prokaryotes, eukaryotes, mitochondria, and viruses.Overlapping genes enable the production of more proteins from a given region of DNA than is possible.

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